Extraction, homogenization, and centrifugation are the three processes in cell fractionation.
Cell fractionation is a method for dividing cellular components while yet maintaining each component's unique functionality. This technique was initially applied to show where distinct biochemical activities are located within cells. Subcellular fractionation is also used to help with illness detection and to offer an enhanced source of protein for further purification. To prevent osmotic damage, tissue is routinely homogenized in an isotonic buffer solution. Grinding, mincing, chopping, pressure changes, osmotic shock, freeze-thawing, and ultrasonic waves are a few of the mechanisms for homogenization. After that, the samples are maintained cool to avoid enzymatic deterioration. It is the development of a uniform mass of cells (cell homogenate or cell suspension).
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It is a soft metal, reactive and with a low melting point. It has an atomic weight of 23 grams. Sodium is a good conductor of electricity due to the presence of delocalized electrons in its metallic structure. It reacts vigorously with water to produce sodium hydroxide and hydrogen
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Summary. Bacteria are like eukaryotic cells in that they have cytoplasm, ribosomes, and a plasma membrane. Features that distinguish a bacterial cell from a eukaryotic cell include the circular DNA of the nucleoid, the lack of membrane-bound organelles, the cell wall of peptidoglycan, and flagella.