Living things in the environment such as plants, animals, and bacteria are biotic factors. Biotic factors also include once-living parts such as dead leaves on the forest floor. Abiotic factors are nonliving aspects of the environment such as sunlight, temperature and water. One important abiotic factor is soil.
concentration of the enzyme, concentration of the substrate, and temperature. However there are more than three. A lock and key analogy is used when explaining the specific action of an enzyme with a single substrate.
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