The answer is _southern blotting_
Hope this helps
OPTIONS:
a. When there are just two other people at the party.
b. When everyone is wearing a nametag.
c. When there are more than 50 people attending the same party.
d. When the need for help from the person who is having a heart attack is very clear
Answer:
c. When there are more than 50 people attending the same party.
Explanation:
Bystander effect is a term used in social psychology to describe the tendency of an individual to intervene in the event of an emergency to offer help to the person needing it, when others are present at the scene of the emergency.
In the case of an emergency just like the scenario stated in the question, where someone develops a heart attack at a dance party, if the party has more people, the slimmer the chance of the person getting help from any of the 50 people at the party, as the presence of others would tend to discourage the any individual from attempting to help the victim having the heart attack.
The situation that would more likely show the bystander intervention effect is <em>"c. When there are more than 50 people attending the same party."</em>
Cannabinoid receptors, located throughout the body, are part of the Endocannabinoid system which is involved in a variety of physiological processes including appetite, pain-sensation, mood, and memory.[1]
Cannabinoid receptors are of a class of cell membrane receptors under the G protein-coupled receptor superfamily. As is typical of G protein-coupled receptors, the cannabinoid receptors contain seven transmembrane spanning domains.[5] Cannabinoid receptors are activated by three major groups of ligands: endocannabinoids, produced by the mammillary body; plant cannabinoids (such as Cannabidiol, produced by the cannabis plant); and synthetic cannabinoids (such as HU-210). All of the endocannabinoids and plant cannabinoids are lipophilic, such as fat soluble compounds.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
They will collapse and shut off the stomatal pore
Explanation:
The guard cells are regulated by the presence of water. When water is present, they become turgid and open up the stomatal pore and when water is inadequate, they become flaccid, collapse and close up the stomatal pore as a result.
<em>If the leaf is left under the microscope for too long, there will be loss of water by evapotranspiration and the guard cell will become flaccid and collapse as a result and the stomatal pore will become closed.</em>
