Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
I can’t see your answer choices, but igneous rocks are made from cooled lava or magma. This is why these rocks are so brittle and grainy. I’ve attached a helpful image to better explain! Good luck!!!
Answer:
Metamorphic rocks are changed by the heat and pressure inside Earth.
Explanation:
Answer:
b im pretty sure
Explanation:
Population density is the average number of individuals in a population per unit of area or volume. For example, a population of 100 insects that live in an area of 100 square meters has a density of 1 insect per square meter.
