Question

eosin Eosin is a red stain and methylene blue is blue. What should be the result of staining a bacterial smear with a mixture of eosin and methylene blue?

Answer 1

Answer:

The result will give a smear background of red color or pink , while the  specimen(cell) will turn blue.This is an example of ROMANOWSKY staining.Its main objective is to increase the contrasts for better view.

Explanation:

Histologically,   staining is the process of  adding colors to the speciemen under  focus who can not be discern with naked here to have increase in contrast  to ensure a well defined view.

Eosin is a staining dye that is acidic in nature, while methylene blue is basic,the reaction mixture will separate  the red  color background of  from the blue color of the cell giving a clear contrast .

This technique is mainly used for

1. blood staining and bone marrow staining (biopses)  for disease detection.

2.malaria detection.

3. for Cerecrospinal fluid and lumber puncture  staining,in cytopathology.

Eviences has  shown  that;the mixture  of eosin and methylene blue do not produce the red/blu coloration, but rather the replacement of the methylene blue with AZURE B

malaria detection