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spin [16.1K]
4 years ago
9

A student plans to analyze a protein sample that he suspects to be made of 4 subunits that are non-identical but similar in mole

cular weights. In his lab he has access to only SDS-PAGE and Agarose Gel Electrophoresis. What best describes the scientific method he should use to analyze the protein?
Biology
1 answer:
Butoxors [25]4 years ago
5 0

Answer:

Agarose gel electrophoresis is the most suitable method for this analysis.

Explanation:

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a procedure used for visualizing and separating proteins according to their molecular weight. The use of sodium dodecyl sulfate and polyacrylamide gel eliminates most of the influence of the tertiary structure and charge, and proteins are separated solely based on polypeptide chain length. So, if the student suspects that the protein sample is conformed of 4 subunits similar in molecular weight, SDS PAGE will likely not separate them.

On the other hand, an agarose gel electrophoresis can separate proteins based on their size AND charge, so, if you suspect that the proteins are not identical but similar in size, the charge factor can be important to separate them in an experiment.

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Which of the following statements is true? Select all that apply. Capillaries have a higher resistance to flow than either arter
Nitella [24]

Answer:

  1. Blood travels at a higher velocity in arteries than in veins.
  2. The volume of blood flow in the arteries and arterioles is greater than the volume of blood flow through the capillaries
  3. Blood pressure is higher in arteries than in veins.
  4. The total cross-sectional area of the capillaries is greater than the total cross-sectional area of the venules and veins

Explanation:

The lumen of arteries is larger than that of arterioles and capillaries. However, even though they have a smaller lumen compared to veins, their walls are thicker to bear the pressure of the blood as it is pumped by the heart to the tissues. Therefore the blood pressure and velocity of blood in arteries (that pump blood away from the heart) is higher than in capillaries and veins. Veins take blood back to the heart from tissues hence this blood is under lower pressure and velocity. This is why veins have valves to prevent the back-flow of blood against gravity.

Due to the large cross-section area of capillaries the blood flow is here is slower hence giving time for blood to linger a bit so there is an exchange of materials between the blood plasma and the interstitial fluids that nourish the cells.

3 0
4 years ago
So, as many awesome facts about rainforests needed if possible! Thanks!
Ratling [72]

Answer: Rainforests only cover around 2 percent the total surface area of the Earth, but really about 50 percent of the plants and animals on the earth live in the rainforest. Rainforests are the forests that receive high amount of rainfall.  You can find rainforests in many countries, not just in South America. They can be found in Alaska and Canada, as well as Asia, Africa and Latin America.  Rainforests are found on all of the different continents, except for Antarctica because it is far too cold there for the environment to be conducive.  There are two different types of rainforests, and they include both temperate and tropical. The tropical rainforests are the ones that are most commonly found around the world. Fact 6: Rainforests help to regulate the temperatures around the world and the weather patterns as well.  A fifth of our fresh water is found in tropical rainforests, the Amazon Basin to be exact.  Rainforests help to maintain our supply of drinking water, so they are critical in the sustainability of the earth.  About 1/4 of natural medicines have been discovered in rainforests.   Within four square miles of tropical rainforest, you will find 1500 flowering plant species, 750 types of trees, and many of these plants can be helpful in combating cancer.  70% or more of the plants that are used to treat cancer are found only in the tropical rainforests on the planet.  Over 2000 types of plants that you find in the rainforest can be used to help aide in cancer treatment because they have anti cancer properties.  The Amazon rainforest is the largest tropical rainforest in the world.   rainforests have actually been analyzed to determine their value in the world of medicine.  Rainforests are threatened each and every day, especially by practices such as agriculture, ranching, logging and mining.  There were around 6 million square miles of rainforest in the beginning, but now because of deforestation, there are really only less than half of that still found in the world.

6 0
3 years ago
If someone could help me figure out how to do these sorts of problems I would really appriciate it​
Pani-rosa [81]

Answer:

The possible genotypes are:

I^AI^B, I^AX^H, I^AX^h, I^BI^B, I^BX^H, I^BX^h, X^HX^H, X^HX^h

<em> good luck, i hope this helps :)</em>

8 0
3 years ago
11. Which structure is found in the inner medulla of the kidney?
matrenka [14]

Answer: Option B) loop of Henle

Explanation:

The longitudinal section of the kidney has two distinct regions: an outer cortex and an inner medulla. The outer cortex has the following parts: glomerulus, bowman's capsule, convoluted tubule; while the inner medulla has the loop of Henle, a U-shaped loop that comes from the proximal convoluted tubule found in the medulla.

8 0
3 years ago
Read 2 more answers
Does hydrogen and helium act almost like a furnace in the sun?
viktelen [127]

Answer:

Yes

Explanation:

Two isotopes of hydrogen, tritium and protium, undergo nuclear fusion in the sun to give helium, neutrons and a tremendous amount of energy. This reaction occurs at the very high temperature found in the sun and yields tremendous amount of energy.

Given the high temperature at which the said fusion reaction occurs, it is safe to say that hydrogen and helium act as a furnace at the core of the sun.

8 0
4 years ago
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