Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
Answer:
Average speed is distance divided by time. Velocity is speed in a given direction. Acceleration is change in velocity divided by time.
Explanation:
The pH scale measures how basic or acidic a substance is, and it ranges from 0 to 14. On the pH scale, a pH of 7 is neutral, less than 7 is acidic and higher than 7 is basic.
Answer:
Afferent arteriolar vasoconstriction decreases blood flow into the glomerulus, which causes the glomerular-capillary blood pressure to decrease, leading to a(n) decrease in the net filtration pressure and a resultant decrease in the GFR.
Explanation:
Pretty self-explanatory.
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