Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Palisade mesophyll cells are closely packed to absorb the maximum light. They are at right angles to the surface of leaf to reduce the number of cross walls. Large vacuole pushes chloroplasts to the edge of a cell. Chloroplasts at edge enable short diffusion path for carbon dioxide and to absorb maximum light.
Hope this helped
Answer:
White petal color is a recessive trait.
Explanation:
Further explanation is posted as an attached file due to technical difficulties.
The haploid male (sperm) and female (egg<span>) sex </span>cells<span>; in </span>plants<span>, formed by mitosis of haploid </span>cells<span> in the gametophyte. ... The multicellular diploid portion of the </span>plant life cycle<span> resulting from the growth, mitosis, and </span>cell<span> division of a zygote. </span>Produces<span>sporangium that store haploid spores. Google*</span>
