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zvonat [6]
3 years ago
6

Please help me now I only have 10 minutes to get this done!!!!!!

Biology
1 answer:
galben [10]3 years ago
4 0

Answer:

F

Explanation:

They share a more recent common ancestor

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Young multicelled organisms usually start out small, then grow in size and increase in complexity. this process is called
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Photosynthesis is a process in which plants prepare food using carbon dioxide, chlorophyll, and water in the presence of sunligh
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Which adaptive management stage helps us understand what objectives are actually met?
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6. (3 pts) After transformation, 40 colonies were present on the plate. What is the transformation efficiency of the competent c
natali 33 [55]

Answer:

Given:

Number of colonies on plate = 40

Amount of DNA used for transformation = 100ng

Volume of competent cells used = 100ul

Total volume = 1ml (1000ul)

Volume plated for growth = 200ul

The amount of cells transformed per 1 µg of DNA is called the transformation efficiency

(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA

Amount of DNA used for plating = 100ng * (200/1000)

                                                      = 100ng * (1/5)

                                                      = 100/5 = 20ng

Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng

               (2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA

Transformation efficiency = 2000 Transformants / ug DNA

                                          = 2 x 10³ Transformants / ug DNA

Explanation:

Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.

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What are two events that can take place for Secondary succession to occur?
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Researched (copied and pasted so use in your own words)

Disturbances such as forest thinning, floods, fire and wind can all lead to secondary succession. Examples of secondary succession are the gradual replacement of old fields by forest or the vegetation recovery and change following wildfire occurrence.
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