This definition above refers to the geographic isolation.
It means exactly what it says in the definition - one species is left behind from the rest of the population, and it stays in that particular location, evolving and changing from the species and population it was once a part of.
In the genetic code, codons made of of three bases specify an amino acid. With three bases, there are 64 possible permutations. With three codons corresponding to STOP codons, this leaves 61 combinations that code for an amino acid. This would require 61 distinct tRNA species were it not for the "wobble base"
