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Semenov [28]
3 years ago
10

_______________, which causes abdominal pain and diarrhea, multiplies rapidly in prepared foods (

Biology
1 answer:
Lyrx [107]3 years ago
8 0
It is most likely Salmonella :)
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In your laboratory journal, describe the two tools of molecular biology that are often described as the scissors and glue for ma
MatroZZZ [7]

The "scissors" of the molecular biology are: DNA Restriction enzymes.

A restriction enzyme is a protein capable of cleaving a DNA fragment at a characteristic nucleotide sequence called a restriction site. Each restriction enzyme thus recognizes a specific site. Several hundred restriction enzymes are currently known.

Naturally present in a large number of species of bacteria, these enzymes have become important tools in genetic engineering.


The "glue" of the molecular biology are: DNA ligase

In molecular biology, DNA ligases are ligase-class enzymes that catalyze the formation of a phosphodiester bond between two segments of DNA. DNA ligases are involved in several essential cellular processes of DNA metabolism: in DNA replication, suture of Okazaki fragments, and in DNA repair and homologous recombination.


The use of these tools in molecular biology: Cloning

Molecular cloning is one of the bases of genetic engineering. It consists of inserting a DNA fragment (called insert) in an appropriate vector such as a plasmid for example. The new plasmid thus created will then be introduced into a host cell, generally the Escherichia coli bacterium. This will then be selected and multiplied to obtain a large amount of the plasmid of interest. Cloning a gene involves inserting it into a plasmid. A clone will be the bacterial transformant that contains this particular plasmid. In this case we speak of clone because all the individuals of the bacterial colony are genetically identical. Molecular cloning is thus different from reproductive cloning (creating an individual genetically identical to another but of a different age) or therapeutic cloning (making tissues from stem cells to perform transplants compatible with the recipient).

Molecular cloning requires restriction enzymes capable of cleaving the DNA, and DNA ligase capable of re-gluing the DNA fragments. Ligase was isolated for the first time from T4 bacteriophage. This enzyme is involved in the repair and replication of DNA. It can bind DNA fragments with compatible sticky ends. At higher concentration, this enzyme is also able to bind two ends of DNA as shown here. T4 DNA ligase works using ATP and Mg ++. It has an activity optimum of 16 ° C, but remains active at room temperature.

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Which pair best matches a type of land cover with an appropriate land use?
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D Pastureland - Grazing Livestock
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Which mantle is under the crust the upper mantle or the lower mantle
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You're right. The Upper Mantle is directly under the Crust. Hope this helped! =^-^=

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Assuming that transcription and translation both proceed from left to right, which is the correct orientation of the DNA templat
aleksklad [387]

The correct answer is:

DNA template 3'– ……… –5'

RNA transcript 5'– ……… –3'

Protein product H2N– ……. –COOH

In nucleic acids, DNA and RNA 5’ end refers to phosphate group attached to the 5′ carbon of the ribose ring, while 3’ end refers to the ribose -OH substituent. Nucleic acids can only be synthesized in the 5′-to-3′ direction, which means that the DNA template is 3’—5’. Translation of the protein by the ribosome will also proceed in a 5′ to 3′ direction, which means that the mRNA template is 3’—5’.

The protein during the synthesis is extend from its N terminus toward its C terminus.

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