Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Pollution is the biggest impact as well as deforestation
The number of sunflower sprouts will be higher than birch tree sprouts because sunflower sprouts have a higher growth rate than birch tree sprouts.
<h3>What is the growth rate?</h3>
Growth rate refers to the ratio of development of a given population and/or biological structure.
The growth rate may result very useful in ecology studies as above mentioned (ecological succession).
In conclusion, the number of sunflower sprouts will be higher than birch tree sprouts because sunflower sprouts have a higher growth rate than birch tree sprouts.
Learn more about the growth rate here:
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In interphase the dna is the form of loose threads called chromatin
