Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
Answer: A. Heat within the Earth
Explanation: the earth’s crust floats on liquid rock. The convection currents caused by the Earth’s rotation cause movement at the surface.
it would be most likely A.
since female sea turtles usually tend to use up mid may to almost august they usually visit more than once since they lay 150+ eggs.
Answer:
b. Infection with a hepatitis virus
Explanation:
The infection with hepatitis virus can cause sever tissue damage that leads to permanent scarring.
