To measure cross-linking amino acids in a collagen hydrolyzate using a conventional amino acid analyzer with ninhydrin detection, the bulk amino acids must be largely removed to avoid plugging the reaction coil. Tissue is thoroughly reacted with sodium borohydride to convert all intermediate cross-linking residues to their reduced, acid-stable forms. More vigorous reduction conditions may be used than when preparing profiles of tritium-labeled cross-links using NaB3H4<span> and direct amino acid analysis. </span>
Suppose that part of an amino acid sequence of a protein changed from tyrosine-proline-glycine-alanine to tyrosine-histidine-glycine-alanine. This change was most likely caused by a point mutation called a <u>substitution</u>.
<u>Explanation:</u>
Any protein is synthesized by joining a number of amino acids together. This amino acid is placed based on the sequences of the DNA , for every 3 sequence there is an amino acid that is synthesized and therefore it is called as a triplet codon, since there may be many sequence for a single amino acid we call it as degenerate codon.
Answer:
the answer would be muscular coodination
and the cardiac muscles do not get rest
