Answer:
Problems with transfection efficiency or absence of essential mRNA modifications (capping and poly-A tail)
Explanation:
Translation is the process of protein synthesis from RNA in which the genetic information encoded in a messenger RNA (mRNA) molecule is translated into a protein sequence composed of amino acids. Translation has three steps: initiation, elongation, and termination. During translation, different elements are required to initiate, continue and complete the process, which include essential molecules such as amino acids, mRNA, tRNAs, ribosomes, energy-carrying molecules (i.e., ATP, GTP), initiation factors (i.e., eIF1A, eIF3, etc) and elongation factors (eEF-2, EIF5A). For example, humans can't synthesize nine essential amino acids (i.e., threonine, tryptophan, phenylalanine, valine, methionine, leucine, histidine, isoleucine, lysine), thereby it is imperative to include these amino acids to synthesize proteins composed of them. On the other hand, it is also fundamental to take into account that the efficiency of transfection of the molecules required during translation, as well as the absence of modifications in the mature mRNA sequence, may alter the process in vitro.
Sugar. Plants take in carbon dioxide, water, and nitrogen to create sugar or edible nitrogen. It’s C
Answer:
. 2C- acetyl Co-A from the link reaction enters the kreb Cycle to combine with 4 Carbon Oxaloacetate to form 6- Citrate
The Citrate forms intermediate Isocitrate, which eventually formed 6-C alpha ketoglutarate.
The alpha-ketoglutarate forms the intermediate succinyl-Co A, which later formed 5C-succinate.
5C -Succinate forms 4C-fumarate, the latter formed 4C-malate- which eventually formed 4C-oxaloacetate.
The 4C of these compounds is fixed, to ensure constant availability of 4C of oxaloacatate for 2C Acetyl -CoA to bind it for the cycle to continuously occur for production of first product Citric Acid from which other products are formed from.
Explanation:
