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Vesna [10]
3 years ago
8

You INCORRECTLY perform an acid-fast stain on a smear from a mixed culture of Mycobacterium smegmatis (acid-fast) cells and Stap

hylococcus aureus (non-acid-fast) cells. Your mistake is that you forget to use methylene blue during the procedure. If you viewed this slide with a microscope, what would each cell type look like?
Biology
1 answer:
Dahasolnce [82]3 years ago
5 0

Answer:

In acid-fast staining, carbon fuchsin is used as a primary stain which dissolves the mycolic acid present in the cell wall of <em>Mycobacterium smegmatis </em>and penetrates through it which results in staining <em>Mycobacterium</em> red.

Staphylococcus aureus cell wall does not contain mycolic acid so carbon fuchsin does not penetrate its cell wall, therefore, it becomes colorless after destaining with acid alcohol.

After destaining step methylene blue is added to stain non-acid-fast bacteria blue. So if I mistakenly forgot to use methylene blue during the procedure <em>Mycobacterium smegmatis</em> will appear red due to carbon fuchsin present in their cell wall and S<em>taphylococcus aureus</em> will appear colorless because it is destained.

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A.The mother's X chromosomes failed to separate during meiosis, and the daughter inherited two X chromosomes with the Lesch-Nyhan mutation. The father contributed no sex chromosomes.

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As seen in the question above, a little girl was diagnosed with Lesch-Nyhan syndrome, which is an X-linked recessive condition caused by a mutation in the HPRT1 gene responsible for purine metabolism.

The little girl's parents do not have the syndrome, and no one in the little girl's paternal family presented this syndrome, however, we know that the maternal grandfather of the little girl's mother had the syndrome, which means that it was the mother's genetic material that contributed to the development of the syndrome in the little girl. This was because the little girl did not receive any X chromosomes from her father, but she inherited the two X chromosomes from her mother that coded for the Lesch-Nyhan mutation. This happened because the mother's X chromosome disjuction did not occur during meiosis I.

As shown above, the father did not contribute any sex chromosomes to his daughter, which means that the daughter is XO and her only X chromosome came from her mother, who was a carrier.

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