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Vesna [10]
3 years ago
8

You INCORRECTLY perform an acid-fast stain on a smear from a mixed culture of Mycobacterium smegmatis (acid-fast) cells and Stap

hylococcus aureus (non-acid-fast) cells. Your mistake is that you forget to use methylene blue during the procedure. If you viewed this slide with a microscope, what would each cell type look like?
Biology
1 answer:
Dahasolnce [82]3 years ago
5 0

Answer:

In acid-fast staining, carbon fuchsin is used as a primary stain which dissolves the mycolic acid present in the cell wall of <em>Mycobacterium smegmatis </em>and penetrates through it which results in staining <em>Mycobacterium</em> red.

Staphylococcus aureus cell wall does not contain mycolic acid so carbon fuchsin does not penetrate its cell wall, therefore, it becomes colorless after destaining with acid alcohol.

After destaining step methylene blue is added to stain non-acid-fast bacteria blue. So if I mistakenly forgot to use methylene blue during the procedure <em>Mycobacterium smegmatis</em> will appear red due to carbon fuchsin present in their cell wall and S<em>taphylococcus aureus</em> will appear colorless because it is destained.

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KiRa [710]

Answer:

See the answer below

Explanation:

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5 mg/ml = 5000 micrograms/ml

There is a need to dilute the stock antibiotic solution in order to arrive at 50 micrograms/ml. Using the dilution principle;

m1v1 = m2v2

5000 x 1 = 50 x v2

v2 = 5000/50 = 1000 ml

<em>Hence, in order to prepare 50 micrograms/ml, 1 ml of the stock Gentamycin should be taken and diluted with 999 ml of distilled sterilized water. 1 ml of the diluted Gentamycin will then be added to each agar plate while they are still in the molten form at a warm temperature.</em>

8 0
3 years ago
How does the structure of Tim O'Brien's story "Ambush" relate to its title?
Nataly [62]

The correct answer is option C, The narrator's platoon was surrounded by the enemy, similar to the way the narrator feels when he is surrounded by his family

Reason -

In the story by Tim O’Brien’s, It is clear that the narrator is puzzled at two instances and at these two instances the word "Ambush" relate to its title The two instances are given below:

a) The first instance is when the narrator’s daughter is asking him question about his war time memories. The narrator feels guilty when he lied to his daughter in order to be a hero in front of her but somewhere he was aware that he was lying.  

b) The second instance is when the narrator threw a bomb and killed a soldier of another nation. He is guilty about killing anyone during wartime.  

4 0
3 years ago
Chromosome.
Mashcka [7]

Answer:

1. x chromosome

2.males

3.one X and one Y chromosome.

4. two X chromosomes

5. a very common trait in humans and frequently used to explain X-linked disorders.[8] Between seven and ten percent of men and 0.49% to 1% of women are affected. Its commonness may be explained by its relatively benign nature. It is also known as daltonism.

6.Females with one copy of the mutated gene are carriers. X-linked inheritance means that the gene causing the trait or the disorder is located on the X chromosome. Females have two X chromosomes while males have one X and one Y chromosome.

Explanation:

4 0
3 years ago
Question 1 of 5
Elden [556K]

Answer:

the correct answer is c

6 0
3 years ago
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Use 2-3 sentences to describe what would if populations never evolved?
s2008m [1.1K]

Answer: Well, populations never evolved than one human might have gone extinct due to many different viruses and sicknesses but other animals might not be extinct.  If populations never grew more then that would be because we could not overpower dome viruses. If we never. If we never evolved animals like a Carrier pigeon would not be dead or extinct today.

8 0
3 years ago
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