The molecular biology technique by PCR promotes, in vitro and by means of temperature variation devices, what the organism naturally performs under physiological conditions: the duplication of DNA strands, involving nucleotides, primers and enzyme polymerases. By this process, it is possible to obtain many copies of a specific nucleic acid sequence from a template tape. This whole process uses the energy of the DNA polymerase itself to create the new copies of DNA.
Each cycle is repeated about 60 times, and promotes amplification of the target region determined by primer affinity. Thus, the primer complementarity recognizes the start site of the site to be amplified, binds, and signals to the polymerase the beginning of the sequence to be replicated.
