Answer:
Polypeptides are composed of amino acids, and we know amino acids are differently charged, have different R groups, and also have different isoelectric points. Depending on different isoelectric points and charged groups, the polypeptides can be separated and because a protein has its lowest solubility on its isoelectric point.
So in this question,
(a) (Lys-Ala)3 ; this is highly positively charged (polar) at pH 7 than (Gly)20 which is uncharged except for the amino and carboxyl terminal.
(b) (Glu)20 ; it is highly negatively charged at pH 7 whereas (Phe-Met)3 is much less polar and hence less soluble.
(c) (Asn-Ser-His)5 ; at pH 3, because in (Ala-Asp-Gly)5 the carboxylate groups of Asp residues are partially protonated and neutral, whereas in (Asn-Ser-His)5 , the imidazole groups of His residues are fully protonated and positively charged.
(d) (Asn-Ser-His)5; at pH 6.0; both polymers have polar Ser sidechains, but (Asn-Ser-His)5 also has the polar Asn side chains and partially protonated His side chains.
Explanation:
Answer: gaps left at the 5' end of the lagging strand.
Explanation:
Eukaryotic Chromosomes are known as the repetitive at the very ends of chromosomes, found in a wide range of Eukaryotic species. They protect the end chromosomes from deterioration or fusion with the neighboring chromosomes.
Telomeres provide a mechanism for their replication by semi conservative DNA replication (a replication in which two parental DNA strands would act as a template for new DNA strands to be synthesized) and length maintenance by Telomerase Enzymes. Telomerase Enzymes are used to extend shortened telomeres during its’ DNA replication.
DNA replication in Eukaryotic Telomeres doesn’t begins at the either end of the DNA strands but starts in the center, and considering that all known DNA Polymerase ( an enzyme that is essential for DNA replication) read the template strand in the 3’ to 5’ direction, one finds a leading strand and a lagging strand on the DNA molecule being replicated.
On the leading strand, DNA Polymerase make complementary DNA strand without any difficulty because it reads the template strand from 3’ to 5’.
On the other hand, there is a difficulty going in the other direction on the lagging strand.
WHY? This is “due to gaps left at the 5’ end of the lagging strand”. To overcome this difficulty, short sequences of RNA acting as Primers (a short single-stranded nucleic acid utilized by all living organisms in the initiation of DNA synthesis) attach to the lagging strand, a short distance ahead of where the initiation site was.
I hope this helps alot!
If a chromatographic column is not placed in a vertical position, the flow and the separation rate of the materials which are been separated may be negatively affected, that is, it may be slowed down, this is because the column is not inclined in the same direction as the flow of the separating substances. Also, collecting the products of the separation will be a bit difficult. If the column is placed in a vertical position, then a test tube can be placed at the end of the column for collecting the separated product
The answer is C; hundreds of years
Answer:
The mRNA sequence that complements the DNA sequence GATCAC is CUAGUG.
Explanation:
A is adenine, which in DNA, pairs up with Thymine.
In mRNA, A pairs up with U, Uracil. Uracil is simply Thymine's replacement in mRNA.
In both mRNA and regular RNA, G, Guanine, and C, cytosine, always pairs up.
To find the sequence, I followed the sequence, but just switched it up with its pairs, getting CUAGUG from GATCAC.
<em>Hope this helped and good luck :)</em>
