Question

The Lineweaver–Burk plot, which illustrates the reciprocal of the reaction rate ( 1 / v ) versus the reciprocal of the substrate concentration ( 1 / [ S ] ) , is a graphical representation of enzyme kinetics. This plot is typically used to determine the maximum rate, V max , and the Michaelis constant, K m , which can be gleaned from the intercepts and slope. Identify each intercept and the slope in terms of the constants V max and K m .

Answer 1

Answer:

• x-intercept = $\frac{-1}{Km}$
• y-intercept = $\frac{1}{Vmax}$
• Slope = $\frac{Km}{Vm}$

Explanation:

Please check the graph attached.