(a) very frequent use of something so that it stops being effective; an over-use.
The answer is iron. Research has shown an increase in serum iron concentreation in athletes and a concomitant decrease in magnesium ions. The increase in iron concentration is thought to be attributed to tissue injury from the strenuous physical activity. The decrease in magnesium is due to demand by the skeletal muscles.
The protists belong to the kingdom Protista that comes under the domain Eukarya. Protists are a very broad group, comprising of various eukaryotes that do not fit into the other kingdoms, like animals, plants, and fungi. The protists were the initial eukaryotes.
They would go on to become the precursors for each of the other eukaryotic kingdoms. By examining protists one can witness the transformation between the single-cell prokaryotes and modern multicellular eukaryotes.
The answer is; Benedict’s reagent
It is used to test for reducing sugars. In presence of these sugars, the reagent, that is blue in color, turns different shaded of red (to brick red), depending on the amount of reducing sugars in the solution. It is important to note that the solution has to be heated.