1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
7nadin3 [17]
3 years ago
15

Restriction enzymes are used in making recombinant DNA. Describe the role restriction enzymes perform when constructing recombin

ant DNA.

Biology
1 answer:
Andrei [34K]3 years ago
6 0

Hi,

Recombinant DNA technology is the process in which a target gene  of interest is replicated by inserting it into a vector and creating a recombinant DNA. This recombinant DNA is then returned into an expression system where it is replicated several times to give rise to multiple copies of our desired gene or that gene product.

One of the very important role for the execution of this technology is performed by Restriction endonuclease enzymes. These are the enzymes that have ability to cut the DNA fragment at specific sequence into fragments of different lengths called restriction fragments.

  • They play a very important role in recombinant DNA technology:
  • They can map the location of specific restriction sites where the target sequence is identified and cleaved.
  • They cut down the DNA at specific sites called restriction sites. The host DNA in which foreign gene or target gene is inserted is also cut with the same restriction enzymes. So in the recombinant DNA technology, restriction enzymes act like a molecular scissor which identify and cut certain DNA sequences as per or requirement.
  • The target sequence can be cut from multiple places by restriction enzymes since our target sequence can be present multiple times in the DNA of organism.
  • When restriction enzymes cut the DNA, they leave sticky or blunt ends at that site in such a way that these ends are able to bind with the complementary bases of vector DNA when introduced.
  • Then another enzyme DNA ligase seals the ends of target DNA and vector DNA making a whole recombinant DNA that is ready to be cloned and progress further steps of recombinant DNA technology.

<em>You can see attached image for better understanding.</em>

Hope it help!


You might be interested in
How do mutations affect natural selection?
never [62]

Answer:C: reproduce

They affect how likely an organism is to survive and

reproduce

Explanation:

6 0
3 years ago
Read 2 more answers
Deforestation is causing the rainforests around the Amazon to dry up
inna [77]
True deforestation causes rainforests around the amazon to dry up
3 0
3 years ago
Question 6 of 10
Marizza181 [45]
The answer should be A.
4 0
3 years ago
Fill in the blanks.
AlexFokin [52]
One of them is definitely hun
7 0
3 years ago
Read 2 more answers
Lesson 5 lesson review<br><br><br> Just help me with whatever you can
lyudmila [28]
9. 2006
10. certain areas in Florida might not receive as much water as other areas that a higher amount of precipitation
6 0
3 years ago
Other questions:
  • Which type of tectonic plate boundary is found between the South American Plate and the Scotia Plate?
    10·2 answers
  • What happens when liquid becomes a gas?
    8·1 answer
  • Which of the following hormones is released by the anterior pituitary as a part of that positive feedback loop between it and th
    12·1 answer
  • You are given two true-breeding groups of rabbits. the first group has white hair. the second group has black hair. the second g
    14·1 answer
  • Which of these organisms is a vertabrate? tulip, horse, slug, or fungus
    8·1 answer
  • Which organisms are in the same kingdom because they cannot form organs?
    13·2 answers
  • When neurotransmitters latch onto the receptors on the ____ of the receiving neuron, tiny gates in the receiving cell's membrane
    10·1 answer
  • Which of these is a statement of opinion?
    5·1 answer
  • Where does transcription take place in the cell
    15·1 answer
  • If a mother has an AB blood type and her daughter has BB blood type, The dad has
    8·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!