Since rifamycin inhibits the enzyme RNA polymerase, which is a key enzyme for synthesizing messenger RNA, the immediate effect of this antibiotic would be the inhibition of the process of transcription.
Messenger RNA is essential for protein synthesis because it is the mediator between the information in the DNA sequence and the process of translation in which the proteins are made. Therefore, by inhibiting RNA synthesis rifampicin ultimately stops the production of proteins.
Is produced in the mitochondria
Answer:
DNA replication a process of copying of a cell's DNA. DNA replication is semiconservative process which means that each strand in the double helix helps in the synthesis of new, complementary strand and conserve the parent template.
The Molecular mechanism of DNA replication is as following:
- The double starnded DN in binded with hydrogen bond, the enzyme helicase opens up the DNA at the replication fork.
- A single stranded binding protein prevent the rewinding of DNA and so binds to the DNA around the replication fork
- Topoisomerase prevent supercoiling at replication fork.
- The ezymes primase come in action and produces RNA primers which are complementary to the DNA strand.
- DNA polymerase III help to extends the primers and allow them to add to the 3' end, to make new DNA.
- DNA Polymerase then remove RNA primers and replace with DNA.
- DNA ligase blocks the the gaps between DNA fragments.
So, this is the molecuar mechanism of DNA replication.
