Answer:
True
Explanation:
There are certain limitations associated with the Punnett Squares. They are not useful in case of complex genetic inheritance such as linkage between two genes. In case of linkages it becomes difficult to estimate the distribution of genotypes and phenotypes. For example in case of Nail-patella Syndrome and gene associated with blood group two genes lie on the same chromosome in close vicinity and hence there are high chances of inheritance of these traits in the offspring from the parent thereby causing random distribution of the two traits. This random distribution cannot be captured through punnet square.
The same problem is associated in case where a single gene is determined by multiple genes with graded effects of each gene.
To find out if it's true or not
Answer:
The observed image will not be sharp
Explanation:
When 100x microscope objective lens is being used, light refraction in the air is enough high to produce image alterations. Image distortions can be minimized by immersion oil because this liquid has a higher refractive index than air. However, light refraction is not usually noticeable when using 4x, 10x and 40x objective lenses. Moreover, this image will not be sharp after putting oil on the slide and going back to the 40x objective, it is for that reason that the 40x is called 'high dry' lens. In consequence, it is required to clean the immersion oil off the 40x objective. In general, it is required to to remove the oil and refocus off the slide and lens immediately after oil immersion (it can be done by using an oil-soluble solvent).
