Answer:
The correct answer is -
a. Protonation of the substrate.
b. Deprotonation of the amino acids in the enzyme active site.
c. Change in tertiary structure in extreme conditions.
Explanation:
During an enzyme assay, suboptimal pH is changed and affect the Vmax to decrease and this could take place due to various reasons such as-
A) protonation of the substrate - Upon protonating or adding a proton or hydrogen cation to a substrate atom, the mass and the charge of the species each increase by one unit which make a conjugate acid.
B) Deprotonation of the amino acids in the enzyme’s active site -Changing the pH will affect the charges on the amino acid molecules.
C) Denaturation of the protein’s tertiary structure- Tertiary structure of the protein is basically a backbone of peptide chain with one or more secondary domains of proteins that increasing the pH or decreasing the pH get denature.
Thus, the correct answer is : option A, B, C.
This is the order;
<span>1.
</span>Extract mRNA from cells
<span>2.
</span>Reverse transcribe mRNA to cDNA
<span>3.
</span>Label cDNA with a chemiluminescent molecule
<span>4.
</span>Add cDNA to microarray and incubate
<span>5.
</span>Wash away unbound cDNA
<span>6.
</span>Visualize microarray and analyze the data
Ingestion<span> - the process of taking food into the body through the mouth (as by eating) consumption, intake, uptake.
</span><span>
</span>
Answer:
to know how many correspond to each tray if we divide the total cookies equally, we need to make a division of the total cookies divided by the amount of trays, that is:
567 cookies / 7 trays = 567/7
= 81
therefore, there will be 81 cookies in each tray
Explanation:
The right answer is D) a structure made of two or more tissues functioning together for a common purpose.
An organ is a set of specific tissues capable of performing one or more specific functions. The human body has 78 organs and these organs can be grouped into systems (there are 11 of them) to perform more complex functions. For example, the bronchi and lungs are organs that belong to the respiratory system. Organs are necessary to live in good health and some organs are essential. These are the brain, heart, lungs, kidneys, liver and pancreas.
