Answer:
Cas9-sgRNA cannot align with targeted DNA sequence
Explanation:
Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.
It is important in determining whether injuries/death was an accident or purposeful, and if purposeful, who, how and why did someone do it. They determine the weapon through the ligature marks left, the shape, force and impact of the injury, the location of the injury, and by analysing any fragments and trace evidence left on the wound
Polyunsaturated fats. The four types<span> have different chemical structures and physical properties. The bad fats, saturated and trans fats, tend to be more solid at room temperature (like a stick of butter), while monounsaturated and polyunsaturated fats tend to be more liquid (like liquid vegetable oil).</span>
The answer is definitely C. Dysfunctional Behaviors.
Yes it could change the park if you remove a population from the national park
