1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Neporo4naja [7]
3 years ago
9

You are conducting a CRISPR-Cas9 experiment. You transform a cell with Cas9 and a guide RNA (contains the scaffold and guide seq

uences), but then realize there is no PAM sequence near your target sequence. What is the likely outcome of this
Biology
1 answer:
SOVA2 [1]3 years ago
8 0

Answer:

Cas9-sgRNA cannot align with targeted DNA sequence

Explanation:

Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.

You might be interested in
Use a specific example to explain how the interactions of two or more body systems work together to perform a function.
alexdok [17]
The sperm and the egg
6 0
3 years ago
Please help me
horrorfan [7]

Answer:

C. But see the answer under C. Apparently C is the accepted answer.

Explanation:

A. Not A.I don't think that's a problem for gulls especially on beaches.

B. Not B.  Their feather do not look like they need much help in keeping their feathers attached when in flight.

C. This is not really the answer, but it is the best answer of these 4. The actual answer is that the bones are porous for better air intake which provides more oxygen and hence they are better able to fly.

D. Not D. Food has nothing to do with the problem. Birds get an adequate amount of food without their wings being porous. See the answer for C.

6 0
3 years ago
Read 2 more answers
Which of these infections is also referred to as Valley fever?
lorasvet [3.4K]

Answer:

coccidioidomycosis

Explanation:

Valley fever is also referred as coccidioidomycosis. It is an acute infection caused by a fungi called coccidioides. The fungi present in the soil in a particular region. The spores of the fungi spread into air through wind, construction work or farming. Through breathing fungi reached to the lungs causing valley fever. The symptoms of coccidioidomycosis includes fever, pain in chest and coughing. Mild valley fever get cured in due course of time by itself but in severe cases antifungal medicines are prescribed by the physicians.  

5 0
4 years ago
Define acidity. How is it measured?
Aleksandr [31]

Answer:

its the level of acid in substances

you put the amount of a item into a cup and add water.

Explanation:

4 0
3 years ago
According to the big bang theory the universe was once a very
kati45 [8]
According to the Big Bang theory, the universe was once very dense and is now expanding. 
8 0
3 years ago
Other questions:
  • What is considered the fundamental unit of life?
    12·1 answer
  • Which of the following practices can help control erosion?
    13·2 answers
  • Difference between inhalation and exhalation???
    10·2 answers
  • The red-billed oxpecher (a bird) eats ticks off the fur of an impala (a type of antelope) the oxpecker received food and removed
    5·2 answers
  • Two dangers associated with the exposure of X-ray
    12·1 answer
  • Explain three ways in which the human<br>sperm cell is adapted to its function.​
    11·1 answer
  • Quais são as meninges que revestem o Sistema Neural Central? Determine a ordem delas no sentido de dentro para fora.
    5·1 answer
  • True/ False: Substances will diffuse more rapidly through a dense substance. Explain
    13·2 answers
  • The Glomar Challenger (Joides Resolution) is known mainly for: a. being the first modern scientific vessel to circumnavigate the
    7·1 answer
  • - Explain the relationship between group behavior, an organism's survival, and the change in a species over time.
    10·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!