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Neporo4naja [7]
3 years ago
9

You are conducting a CRISPR-Cas9 experiment. You transform a cell with Cas9 and a guide RNA (contains the scaffold and guide seq

uences), but then realize there is no PAM sequence near your target sequence. What is the likely outcome of this
Biology
1 answer:
SOVA2 [1]3 years ago
8 0

Answer:

Cas9-sgRNA cannot align with targeted DNA sequence

Explanation:

Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.

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The glucose concentration is higher in the cytosol of an intestinal epithelial cell (IEC) than it is in the intestinal lumen and
monitta

Answer:

The correct option is b. "IEC Na+/glucose symporters are found only in the plasma membrane (PM) facing the basal extracellular fluid"

Explanation:

The glucose in the intestine is a product of digestion and needs to be absorbed into the blood so it can be transported to where needed. Glucose moves out of the IEC with the combined action of sodium ions. The sodium-glucose symporter opens and allows for this pair to move out only when one of each is attached to the symporter. The movement of glucose is regulated in this way.

7 0
3 years ago
Circadian rhythm refers to
strojnjashka [21]

Answer:

The correct option is B) a pattern of biological functioning that occurs on a 24-hour cycle.

Explanation:

Biological rhythm is defined as the regular variation of an organic function related to the course of time, circadian biological phenomena (around the day), are those with a period of approximately 24 hours.

Circadian rhythms are physical, mental and behavioral changes that follow a daily cycle (the biological rhythm), and that respond primarily to light and darkness in an organism's environment. This cycle close to 24 hours, allows to recognize the sleep-wake phenomena and their homeostatic action, in the systemic processes of every living being. The circadian system is composed of photoreceptors, pacemakers and efferent pathways that allow a regular functioning of the sleep and wakeful state.

5 0
3 years ago
Rhizopods use cilia for locomotion. <br> a. True<br> b. False
NISA [10]
The answer to the question is true
4 0
3 years ago
Four functions of the __________ tract include ingestion, digestion, absorption, and elimination
jeka94
I believe the correct term to fill in the blank would be digestive. Four functions of the digestive tract include ingestion, digestion, absorption, and elimination.The digestive tract group of organs that works together to convert the food taken in into energy and nutrients needed by the body.
5 0
3 years ago
A geneticist discovers a new mutation in Drosophila melanogaster that causes the flies to shake and quiver. She calls this mutat
Harrizon [31]

Answer:

1. First calculate the distance of the map for trace (eg) and spastic mutation (sps); Recombinant frequency:

vg + sps + vg sps + = 99 + 97 = 196

we are going to divide 196 by 650 (total descendants) = 0.3.

in the next step we are going to calculate the recombinant frequency 0.3 * 100 = 30 mu or cM or 30%

this shows that there is an association between vestige (eg) and spastic mutation (sps) since the recombinant frequency is less than 50%.

The squareness test is not valid because the combining frequency is far from 50%.

2. Results:

1. Genes are linked.

3. The test rejected the independent assortment law because it is not true that there is a 1: 1: 1: 1 relationship between descendants

8 0
4 years ago
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