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Neporo4naja [7]
3 years ago
9

You are conducting a CRISPR-Cas9 experiment. You transform a cell with Cas9 and a guide RNA (contains the scaffold and guide seq

uences), but then realize there is no PAM sequence near your target sequence. What is the likely outcome of this
Biology
1 answer:
SOVA2 [1]3 years ago
8 0

Answer:

Cas9-sgRNA cannot align with targeted DNA sequence

Explanation:

Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.

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What is the point of DNA replication​
Degger [83]

Answer:

DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. Replication is an essential process because, whenever a cell divides, the two new daughter cells must contain the same genetic information, or DNA, as the parent cell

Explanation:

DNA replication is the biological process in which two identical copies of DNA are produced from one original DNA molecule.

The steps involved in the process of DNA replication are as follows:

The first step in DNA replication is to ‘unzip’ the double-helix structure of the DNA molecule.

This is carried out by an enzyme called helicase which breaks the hydrogen bonds holding the complementary bases of DNA together (A with T, C with G).

The separation of the two single strands of DNA creates a ‘Y’ shape called a replication ‘fork’. The two separated strands will act as templates for making the new strands of DNA.

One of the strands is oriented in the 3’ to 5’ direction (towards the replication fork), this is the leading strand. The other strand is oriented in the 5’ to 3’ direction (away from the replication fork), this is the lagging strand. As a result of their different orientations, the two strands are replicated differently.

A short piece of RNA called a primer (produced by an enzyme called primase) comes along and binds to the end of the leading strand. The primer acts as the starting point for DNA synthesis. DNA polymerase binds to the leading strand and then ‘walks’ along it, adding new complementary nucleotide bases (A, C, G and T) to the strand of DNA in the 5’ to 3’ direction. This replication is continuous. Numerous RNA primers are made by the primase enzyme and bind at various points along the lagging strand.

Chunks of DNA, called Okazaki fragments, are then added to the lagging strand also in the 5’ to 3’ direction. This type of replication is called discontinuous as the Okazaki fragments are joined up later.

Once all of the bases are matched  (A with T, C with G) an enzyme called exonuclease strips away the primer(s).

The new strand is proofread to make sure there are no mistakes in the new DNA sequence.

Finally, an enzyme called DNA ligase seals up the sequence of DNA into two continuous double strands.

The result of DNA replication is two DNA molecules consisting of one new and one old chain of nucleotides. This is why DNA replication is described as semi-conservative, half of the chain is part of the original DNA molecule, half is brand new.

Following replication, the new DNA automatically winds up into a double helix

(b) DNA replication occurs in S phase of cell cycle in eukaryotes. If cell division is not followed after DNA replication then the replicated chromosomes (DNA) would not be distributed to daughter nuclei. A repeated replication of DNA without any cell division results in the accumulation of DNA inside the cell nucleus, leading to polyploidy.

7 0
2 years ago
Will mark Brainliest
zubka84 [21]

Answer:

c

Explanation:

5 0
2 years ago
Hermit crabs have a soft belly and live in the ocean. To protect themselves, they crawl into discarded snail shells and use them
aniked [119]
The relationship between  the  hermit crabs and the snails  is  A.commensalism

Why? Commensalism refers to a relationship where one organism benefits  from the other without  affecting it. In this case, the hermit crab benefits from the  snails, via the latter's discarded shells. The crab's use of the  snails' shells has no impact on the  snails.
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Is the fins are for breathing?<br>​
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Answer: yes

Explanation:

Yes

4 0
2 years ago
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Which of the following is not a similarity between green algae and plants
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