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Neporo4naja [7]
3 years ago
9

You are conducting a CRISPR-Cas9 experiment. You transform a cell with Cas9 and a guide RNA (contains the scaffold and guide seq

uences), but then realize there is no PAM sequence near your target sequence. What is the likely outcome of this
Biology
1 answer:
SOVA2 [1]3 years ago
8 0

Answer:

Cas9-sgRNA cannot align with targeted DNA sequence

Explanation:

Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.

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What is a european starlings ecological role
makkiz [27]
European Starlings have a significant impact on their environment because they congregate in such large numbers.  They probably play a role in seed dispersal because of their consumption of a wide variety of fruits.  European Starlings also control some insect populations, but since they will eat almost anything they cannot be relied upon to eat only pests.  Insects they are known to feed on include the larvae of craneflies (Tipulidae) and moths (Lepidoptera) as well as mayflies (Ephemeroptera), dragonflies and damsel flies (Odonata), grasshoppers (Orthoptera), earwigs (Dermaptera), lacewings (Neuroptera), caddis flies (Trichoptera), flies (Diptera), sawflies, ants, bees and wasps (Hymenoptera) and beetles (Coleoptera).  They will also eat small vertebrates such as lizards and frogs, as well as snails (Gastropoda) and earthworms (Annelida).  European Starlings have a particular technique of inserting their closed bill into the ground or an object and then prying the bill open, creating a small hole.  This allows them to forage efficiently in soil and among roots as well as in feed troughs and on the backs of ungulates where they search for ectoparisites.
5 0
3 years ago
The mineral crystals in obsidian are too small to see without a microscope. This is because it _____.
Serjik [45]
<span>The mineral crystals in obsidian are too small to see without a microscope. This is because the rock cools so rapidly from magma that crystals barely have time to form. Obsidian is a normally happening volcanic glass framed as an extrusive molten shake. It is delivered when felsic magma expelled from a well of lava cools quickly with negligible precious stone development.</span>
7 0
3 years ago
Read 2 more answers
Help………………………………………………………
natta225 [31]
What’s the question?
5 0
2 years ago
Match the following
Brilliant_brown [7]

Answer:

Matches according to the terms

Explanation:

1. Stratosphere: The layer of the atmosphere that the ozone layer is part of

2.Recycle:  the process by  which certain waste  materials, such glass, aluminum, and paper are  prepared for reuse

3. Hazardous: the type of solid, liquid, or gas wastes that are      

   toxic,flammable, corrosive, reactive, or radioactive

4. Biodegradable: The type of wastes that can be broken down by other organisms into a reusable form

5.Nicotine: The addictive drug found in tobacco

6. Cancer: A disease frequently developed by smokers and passive smokers.

7.Diabetes:  A disease of the pancreas in which insulin is not produced in

The correct amounts so that glucose can be converted into

energy or stored as fat (insulin not given in the option)

8.Ozone: Organic compounds (VOCs), and nitrogen dioxide (NO) are

acted upon by sunlight

9.Plastic: When burned, it can cause an increase of CO2, ozone, and  sulfur dioxide in the atmosphere

10. Fossil fuel: Sulfur dioxide,aluminium,

11.Ozone: formed in the atmosphere when chemicals, called volatile

7 0
3 years ago
Hello?? Can anyone help me??! PLEASE
kolezko [41]

Answer:

The temperature is staying the same. In the graph when it shows solid/liquid and liquid/gas, the temperature stays the same until it changes. This is because it reached it's melting point/vaporizing point. For example, a solid gets heated up, it then reaches it's melting point but it can't go higher than that because it isn't fully a liquid yet, once it's a liquid it will then continue to rise in temperature.

I don't think I put enough detail into that explanation but I hope this helps your problem.

4 0
2 years ago
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