1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Neporo4naja [7]
3 years ago
9

You are conducting a CRISPR-Cas9 experiment. You transform a cell with Cas9 and a guide RNA (contains the scaffold and guide seq

uences), but then realize there is no PAM sequence near your target sequence. What is the likely outcome of this
Biology
1 answer:
SOVA2 [1]3 years ago
8 0

Answer:

Cas9-sgRNA cannot align with targeted DNA sequence

Explanation:

Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.

You might be interested in
Which of the following is an example of active transport in a cell?
denis23 [38]

Answer:

the answer is D :)

Explanation:

Active transport uses energy molecules to move particles like sodium, against their concentration gradients, from areas of low concentration to areas of high concentration.

8 0
3 years ago
Which food molecule (monosaccharide, polysaccharide, lipid, or protein) would you eat if...
torisob [31]
<span>You needed a quick boost of energy - MONOSACCHARIDE

You wanted to grow strong nails, you haven't eaten in days - PROTEIN

You wanted to grow healthy hair - PROTEIN

You had a race tomorrow afternoon - POLYSACCHARIDE 

You were getting ready for hibernation - LIPIDS

You wanted to get bigger muscles - PROTEIN 

Your meal will be in a week - LIPIDS

CARBOHYDRATES include monosaccharide and polysaccharides. They provide immediate energy boost or short term energy storage for animals as well as long term energy storage for plants. 

PROTEIN s</span>peeds up chemical reactions by lowering activation energy. It is used to form bones and build muscles. It is m<span>ade up of an amino group, a carboxyl group, and an R group.

LIPIDS can be saturated or unsaturated fatty acids. They are not soluble in water however they provide long-term energy storage for animals. </span>
7 0
3 years ago
Faciliatates the process of DNA Replication by unzipping the base pairs
gizmo_the_mogwai [7]
So understanding<span> how the </span><span>DNA </span>molecule behaves inside cells<span> helps explain how genetics works</span><span> at the simplest level. </span>
3 0
4 years ago
For which patient will the nurse need to consult the beers criteria prior to administering medications?
IgorLugansk [536]
<span>The nurse would need to consult the beers criteria prior to giving medications to patients who fall in the category of elderly. This list would notify the nurse what medications are considered inappropriate. Incidentally, the list of patients not covered by this list include newborns, toddlers, and teenagers.</span>
6 0
4 years ago
How could natural selection affect humans?
mario62 [17]

Answer:

Explanation:

an example is the ability to endure the sugar, lactose, in milk. In many places of the world, people can't drink milk in light of the fact that their body turns off the intestinal creation of lactase, a chemical that processes the sugar in the milk.

4 0
3 years ago
Read 2 more answers
Other questions:
  • Is a fat replacer used in food processing that can bind to fat-soluble vitamins and thereby reduce their absorption?
    10·1 answer
  • Scientists believe that four-legged mammals might be distant ancestors of modern-day whales. What evidence seems to support this
    8·1 answer
  • Why are viruses not able to make their own proteins
    7·2 answers
  • The table below provides the number of chromosomes found in the body cells of various types of organisms.
    8·2 answers
  • Insects are usually _______.
    13·1 answer
  • Frogs go through metamorphosis.<br> a. True<br> b. False
    9·1 answer
  • Sam wants to demonstrate how water changes from a solid to a gas. He places ice in a pot on a stove. What variable is causing wa
    7·1 answer
  • Proteins that are going to be used within a cell would be synthesized by
    9·1 answer
  • What are the four classes of nutrients?
    13·1 answer
  • What did charles darwin observe on the galapagos islands.
    11·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!