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Neporo4naja [7]
3 years ago
9

You are conducting a CRISPR-Cas9 experiment. You transform a cell with Cas9 and a guide RNA (contains the scaffold and guide seq

uences), but then realize there is no PAM sequence near your target sequence. What is the likely outcome of this
Biology
1 answer:
SOVA2 [1]3 years ago
8 0

Answer:

Cas9-sgRNA cannot align with targeted DNA sequence

Explanation:

Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.

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