Answer:
The correct option is option A
Explanation:
Restriction enzymes are endocleases that cleave DNA fragment (<u>of usually four, five or six nucleotide long</u>) at <u>specific sites to produce blunt or sticky ends</u>. They <u>recognize palindromic sequences of host DNA when cleaving the specific sites</u>. The sequences below (on complementary strands) give an example of a palindromic sequences.
5'-CCC║GGG-3'
3'-GGG║CCC-5'
As can be seen above, when read from 5' to 3', the two sequences are the same despite being on opposing strands. And when cut between the guanine (G) and cytosine (C) (as shown above), it produces a blunt end. But when cut as shown below produces a sticky end.
5'- G║AATTC -3'
3'- CTTAA║G -5'
The explanation above shows options C and D are right while option A is wrong (hence the correct option).
Also, bacteria prevent their own DNA from been digested by restriction enzymes by adding methyl group to their restriction sites <u>which prevents restriction enzymes from recognizing restriction sites of their DNA;</u> this generally makes bacterial DNA to be highly methylated. This explanation makes option B right also.
HELP ME PLES
PERIOD, FREQUENCY OR AMPLITUDE
1. Doesn't change period
2. More of this means more energy
3. Increases as a pendulum swings back and forth faster
4. Measured in cycles per second
5. Measured in meters or centimeters
6. This is decreases with smaller swing
7. If the frequency increases, this decreases
8. Measured in Hertz
9. Measured in seconds
10. if it swings back and forth slower, this decrease
11. As it dampens, this decreases
Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
DNA: TTC. AAT. GGT. CTA. GGG
Com RNA: AAC UUA CCA GAU CCC
Amino Acid: (ASN) (LEU) (PRO) (ASP) (PRO)
The study of living things or the study of life. "Bio" meaning life. "-ology" meaning the study of.
