Answer:
In acid-fast staining, carbon fuchsin is used as a primary stain which dissolves the mycolic acid present in the cell wall of <em>Mycobacterium smegmatis </em>and penetrates through it which results in staining <em>Mycobacterium</em> red.
Staphylococcus aureus cell wall does not contain mycolic acid so carbon fuchsin does not penetrate its cell wall, therefore, it becomes colorless after destaining with acid alcohol.
After destaining step methylene blue is added to stain non-acid-fast bacteria blue. So if I mistakenly forgot to use methylene blue during the procedure <em>Mycobacterium smegmatis</em> will appear red due to carbon fuchsin present in their cell wall and S<em>taphylococcus aureus</em> will appear colorless because it is destained.
Answer:
Sustainable use of the environment
Explanation:
Be it the ecosystem or the food you eat, if you just take up whatever you need instead of over exploiting the resources, another life can benefit from it. The relationship we need to form with our environment should be mutualism or commensalism but not parasitism.
The global distribution of organisms and the unique features of island species reflect the evolution and geological change. Fossils. Fossils document the existence of now-extinct past species that are related to present-day species.
A: Cultural Controls
C: The Scientific Method
