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3 years ago

Name two conditions that must be met for a reliable Gram staining result.

Biology

1 answer:

Hatshy [7]3 years ago

4 0

Answer:

The two important conditions that must be met before gram staining are-

1. The age of the culture should not exceed 24 hours.

2. Over heating, decolonization should be avoided.

Explanation:

Gram staining is a technique developed to distinguish the bacteria on the basis of the composition of the cell wall around the bacteria.

The gram stain is a sensitive technique so must be performed carefully. The precautions should be taken to avoid any error while gram staining that is-

1. Age of the culture- The culture should be freshly prepared just 18-24 hours before the experiment as above 24 hours bacteria lose their ability to retain the color so one would not be able to differentiate between the cultures.

2. While performing the experiment one should avoid overheating and decolorization with ethanol as both the factors lead to the loss of crystal violet stain from the cell and they take pinkish color after safranin staying.

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How many people get cancer per year?

Anna [14]

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Explanation: Have a brilliant day of learning! -Lily ^-^

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3 years ago

BRAINLIEST!<br> Define procrastination and list 4-5 ways that you combat procrastination.

julsineya [31]

Explanation:

The action of delaying or postponing something is called procrastination.

4-5 ways that you combat procrastination are listed below :

Getting rid of distractions
Reward yourself
Schedule a time for doing task
Don't be fearful for doing badly at task
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2 years ago

A group of six students has taken samples of their own cheek cells, purified the DNA, and used a restriction enzyme known to cut

prohojiy [21]

Answer:

D. The two students who have two fragments have one restriction site in this region.

Explanation:

The DNA samples from the cheek cells were subjected to digestion with a restriction enzyme. This enzyme is an endonuclease and cuts the DNA at a specific sequence only. This sequence is called a restriction site. If the restriction site is not present in the sample DNA, the restriction enzyme cannot cut it. The presence of one restriction site in the sample DNA would cut it into two DNA fragments.

Similarly, the presence of two restriction sites in each DNA molecule would obtain a total of three DNA fragments per DNA molecule.

3 0

3 years ago

A castrated female cattle is called?

Keith_Richards [23]

Hello,

your answer would be:

First-calf heifer.

Brainliest!?!

Hope this helps!

Have a great day:)

~Rendorforestmusic

6 0

3 years ago

Up to what concentration is carbon dioxide a limiting factor for photosynthesis in low light intensity?

Mariulka [41]

Answer:

<u>Carbon Dioxide limits photosynthesis at a concentration of around 400 ppm (ambient concentration) in low light.</u>

Explanation:

Limiting factors affect the photosynthetic rate, decreasing the potential amount of carbohydrate made via photosynthesis. Carbon dioxide is essential to this form of biosynthesis- it provides the inorganic carbon that is integrated into carbohydrate molecules.

Carbon dioxide is typically present in the atmosphere at concentrations of 0.04%. Light intensity typically cause stomata to open, and increase CO2 uptake. Higher concentrations of CO2 up to 0.1%, cause dramatic increases in the photosynthetic rate.

Low light, thus means less stomatal opening, and less CO2 uptake. As low light intensity can be limiting, it lowers the concentration of Carbon dioxide at which the rate becomes constant.

8 0

3 years ago