Answer:
1. They have to be able to reproduce, either asexually or sexually.
2. They have to be able to respond to stimuli.
3. They have to have cells, either as a unicellular organism or as a multicellular organism.
4. They have to grow and develop.
5. They have to have homeostasis. This means that they can maintain their own body temperature. Think of this as a thermometer for the body.
6. The last thing is that all living things also have to be able to do is move.
Explanation:
It is the stalk of the plant
your answer is data asasas
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
