Question

Put the following steps of dna replication in chronological order. 1. Single-stranded binding proteins attach to dna strands. 2. Hydrogen bonds between base pairs of antiparallel strands are broken. 3. Primase binds to the site of origin. 4. Dna polymerase binds to the template strand. 5. An rna primer is created.

Answer 1

The correct order is:

2. Hydrogen bonds between base pairs of antiparallel strands are broken  

Enzymes called helicases are responsible for this process because their role is to separate two DNA strands so they can be replicated.

1. Single-stranded binding proteins attach to DNA strands.  

After two strands are separated into a Y shape-replication fork single strands are free for the replication machinery. Single strands are the templates for replication to begin.

3. Primase binds to the site of origin.  

Primase is an enzyme which functions as RNA polymerase and thus catalyzes the synthesis of a short RNA segment-RNA primer.

5. An RNA primer is created.

Primer RNA is short RNA sequence that initiates DNA synthesis because DNA polymerase is disable to initiate polynucleotide synthesis by itself.  

4. DNA polymerase binds to the template strand.

DNA polymerase binds to the strand at the site of the primer. The function of this enzyme is to add new nucleotides complementary to other strand.