In an experiment discussed in class, Francis Crick isolated revertants of the mutation FC0. FC0 is a ( ) frameshift mutation in
the rIIA cistron of phage T4. Most of the isolated revertants resulted from second site mutations within the rIIA cistron. One of these second site mutations was called FC10. Crick then separated away FC10 from FC0 and found that FC10 had an rII- phenotype. He then selected for phenotypic revertants of the FC10 mutation and recovered a second site suppressing mutation that he called FC63. Is FC63 a ( ) frameshift mutation or a (-) frameshift mutation.
The correct option is that its a ( ) frameshift mutation, just like the FC0 mutation.
Explanation:
Frame-shift mutations refer to an event of insertion or deletion at the level of DNA sequence. This effects the way (i.e., in what frame) the genetic code is read. These kind of mutations result in producing a dysfunctional protein product., which may be longer or shorter in length to the actual normal protein that was supposed to be made. A premature stop codon will create a truncated protein.
