Spearate cell into the protein cells
Answer:
A. The phenotypes that result from having 2 or 4 dark-skin alleles
Explanation:
Answer:
The gene of interest from another source.
Explanation:
A plasmid is a small, circular double-stranded extrachromosomal DNA molecule within a cell particularly a bacteria cell that is physically separated from chromosomal DNA and can also replicate independently.
Expression vector is usually a plasmid (or a virus can also be utilized) constructed to allow for gene expression in cells. The vector is used to introduce a gene of interest into a target cell, and can drive the cell's mechanism for protein synthesis to produce the protein encoded by the gene, thus giving rise to the expression of the gene.
This gene of interest is integrated into the plasmid that is cut by endonucleases and then ligated using DNA ligase. Thus the expression differs from the normal plasmid in that it contains an external gene sequence.
<u>Answer</u>:
1. The purpose of the uninoculated control tubes used in the oxidation fermentation test is to serve as basis of the medium for the color comparison and that it is sterile.
2. It is necessary to use two controls in order to illustrate the no color change (green color) under both aerobic and anaerobic conditions.
3. The purpose of the experiment is to determine if the organisms utilize the carbohydrate and thus is fermentative. This will result in a color change from green to yellow.
Thus, the medium in one tube is sealed with a layer of paraffin to prevent diffusion of oxygen. This tube will show that the medium is unaffected by the lack of oxygen.
The second tube will allow the diffusion of oxygen. This tube will show that the medium does not change color naturally in the presence of oxygen. Thus, the color changes will be caused only by the fermentation process of the organisms.
