A little more context would be helpful, but in general Gram Staining helps us to decipher between two major groups of bacteria (Gram positive and Gram Negative). This is helpful because different antibiotics must be used to treat infections from the different types of bacteria, so it will help them to properly medicate Anna.
Cuz of the refraction of light
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i don't really know what you're asking. But, if you're asking what the main energy source is then it would be the sun.
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