DNA replication goes in the 5' to 3' direction because DNA polymerase acts on the 3'-OH of the existing strand for adding free nucleotides. DNA replication can take place both the directions chemically. ... But these nucleotide triphosphates hydrolyze spontaneously under aqeuous conditions.
Evaporation
Explanation:
Evaporation is one change that would cause more water to evaporate from a stream.
Evaporation is a phase change in which liquid is changed into its gaseous form.
- When a substance is heated from solid to liquid, it melts.
- After melting, additional heat causes a phase change that allows for the liquid to change to gases.
- In the water cycle, the sun provides heat energy that causes surface water in streams to be heated.
- This leads to evaporation of the surface water into the atmosphere where they can be condensed to form precipitation.
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Answer:
Short answer is primers are partially complementary.
Explanation:
Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3'
Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3'
When we write reverse primer 5' to 3' we can easily see that
3'-TTGGCCAATGG---5' is complementary to the forward primers'
5'---AACCGGTTACC-3' sequence. So instead of binding to the template DNA these primers might bind each other resulting with reduction of efficiency of DNA amplification.
Answer:
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Explanation:
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