The correct answer is option D, Temporal isolation
Temporal isolation is the mechanism which prevents the mating of closely related species living in the same ecosystem. Basically the reproductive barriers prevent these species from interbreeding. Temporal isolation is a prezygotic barrier that causes “time isolation” between the breeding period of two closely related species. The difference in time could be “difference in time of day”, “ difference in season of mating”, “difference in months” and in some cases “ difference in years”.
Is there a picture or do you want me to explain
Inbreeding is increased due to the small number of individuals in the population. Selective breeding; > Humans can cause a decrease in genetic diversity too. ... > Selective breeding involves choosing which animals or plants to breed so that they have certain benefits.
I am not entirely sure what you mean by "responding variable" but I'm going to guess it was the vinegar since it has been used as a perservative for years.
Hope that helps in some way.