Step one: Travel approximately 18 inches up the underside of the branch you are removing. Cut up about halfway through the branch.
Step two: Move to the top side of the branch. Choose a location an inch further out from your first cut. Carefully cut down until the branch breaks free.
Step three: Find the branch collar on your trunk. This is stem tissue around the base of the branch. . Make a complete cut with a 45-degree angle kicking out from the base of the tree.
(SOURCE Chris Lambton) Add your vocabulary words in along the way if you decide to use my answer.
Answer:
E.coli is a bacterium which is preffered for transformation due to its rapid growth and high efficiency of introduction of DNA molecules into cells.
Tetracycline is an antibiotic used to treat several infections such as cholera, plague, brucellosis, and malaria etcetra.
Plasmid is a small, extrachromosomal DNA molecule present in cell which is seprated from chromosomal DNA and have ability to replicate independently.
When a plasmid resistant to tetracycline and kanamycine is inserted into E.coli, the process is called transformation. E.coli might not be able to survive due to absence of plasmid but the transformed strains having antibiotic tetracycline and kanamycine will probably be able to survive it.
Hence, the culture of bacteria carrying plasmid will be able to survive only.
Do u have the diagram ?????
