<span>The pH of the matrix increases and the pH of the inter membrane cell decreases
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The reason behind this is the presence of the electron transport chain within the mitochondria which requires the transport of hydrogen ions. The movement of hydrogen ions results in varying pH. If the mitochondria dies, the hydrogen ions will not move into the matrix, causing its pH to increase, and they will build up in the inter membrane causing a decrease in pH.
scientists can test differences of the area with pigmentation by observing which is lighter and which is darker however they use microscope to look the difference of the pigmentation over the cells.
Go to reddit, r/askteengirls or r/teenagers
You'll get better answers there
The force pushing against an area or surface