Question

List the quality control measures taken to ensure contamination does not occur during the Chelex extraction process (DNA extraction) and give reason

Answer 1

Answer and Explanation:

To promote a good DNA extraction through the Chelex extraction protocol, it is important to follow some measures, which are essential for the entire process to take place efficiently.

The most important measure is to avoid contamination. In this case, all surfaces, container and substances used must be properly sterilized, preventing unnecessary elements from coming into contact with the DNA sample or the protocol reagents.

It is important that the researcher is wearing clean, appropriate clothing and gloves when using this protocol. This avoids impurities and contamination with the researcher's DNA.

Another precaution must be taken with the DNA sample and the reagents that will be used during the extraction. All must be stored correctly, preventing their degradation, especially the DNA, which is a very unstable molecule.

Finally, the researcher must strictly follow all the steps of the protocol as indicated.