Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
The answer is copper. Nonrenewable resources are those that cannot be readily/naturally replaced at rates that match those of consumption (an aspect that allow renewable resources to be sustainable). Copper are made deep in earth at very slow rates hence do not readily renew themselves. Organisms, on the other hand die, and are naturally replaced by offspring.
Answer:Causes for food security
Unemployment can also negatively affect a household's food security status. High unemployment rates among low-income populations make it more difficult to meet basic household food needs.
Food Insecurity
Economic Stability.
Education.
Health and Health Care.
Neighborhood and Built Environment.
Social and Community Context.
Explanation: i DIO! would like brainly for this answer
<span>The answer to this question is the second option-" carbohydrate polymers formed as water is removed". In the dehydration synthesis, polymers are created. This is when two molecules formed a new product which results the loss of water. So, this is a process of forming or making a new compound together with the removal of water.</span>
Answer:
The answer is Cryptogams and Phanerogams.
Explanation:
- <u><em>The kingdom Plantae is divided into two subkingdoms: Cryptogamae and Phanerogamae</em></u>
