3.We anticipate our recombinant DHFR protein to be functional for use in future research applications. However, if you had been
able to test functionality and found that your recombinant GST-DHFR-His protein was not functional, what would you propose is one potential issue you would investigate to troubleshoot further. In other words, what is one thing that could have gone wrong to result in non- functional protein from the method used and results obtained this semester
In the given case, the protein would have required to go through the process of post-translational modification, however, the mentioned protein would have been cultured in the prokaryotic system, the process of post-translational modification would have failed to take place. This step would be essential for the functionality of the protein. Thus, there is a need to culture it within the eukaryotic system.
The covalent and generally the enzymatic modification of proteins post its biosynthesis is termed as post-translational modification.
Antidiuretic hormone that is synthesized and produced in the brain by the hypothalamus, is secreted from the pituitary gland, where it is stored. This hormone is released into the blood stream in response to changes in the water balance in the blood. This hormone helps the kidneys to control and regulate the amount of water in the blood
The RNA codon will be GAU, pairing Cytosine-Thymine-Adenine DNA codon with Guanine-Adenine-Uracil RNA codon. This codon is the Amino Acid, Aspartic Acid. In this codon we see Uracil which is the counterpart of Thymine in the RNA.
