Answer:
Disulfide linkage/bond
Explanation:
The tertiary and quarternary structure of the protein is stabilized by Disulfide linkage which is formed between two thiol groups in the protein. 2-mercaptoethanol is a reducing agent that breaks this disulfide bond.
A protein becomes denature when it loses its native configuration and becomes inactive. So as 2-mercaptoethanol breaks disulfide bond in protein it looses its native configuration and becomes denatured. 2-mercaptoethanol is used in SDS-PAGE to separate protein subunits. Therefore the correct answer is Disulfide linkage/bond.
It makes the proteins ................
A person would have to consider Harriet' s upbringing in that time and imagine all the wrong doing she has seen regarding her parents care; therefore, witnessing her own care as a slave as well. It's no wonder that eventually, one would desire they're own true freedom.
