Increased exposure to X-rays
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer: because of the pandimic
it startExplanation: coronvires is the probelm over 4 years it started in 2019 to 2022 it even more year well never know.
Marine biologists study changes in the ocean. so they will look at the chemical composition of deep waters and see how consecrated the oxygen is. They will also study the movements of the plates because when planted move it usually makes new food for deep life
they will also check animal concentrations in certain areas.
Another thing they do is track animal movements. this could be by putting a tracker on a whale or a shark or anything and watching its depths it goes or watching the distance it goes in a day.
