Without genetic variation there would not be evolution. genetic variation sometimes is a result of a change in the environment. some variation occurs to help the animal better survive in their habitat. maybe there was a disruption in their ecosystem and their species will become vulnerable unless they change somehow
Since there’s nothing here other than what you’ve asked i’ll guess, an experiment that is more likely to be reliable will not use the first values it gets, will be done multiple times and the values will be close in number
If you go on mc3.edu/admissions/applying-to-mccc/testing-and-assessment/assets/biology-placement-test.pdf page 11 it will tell you all the answers to your questions....
Severe low back pain and bruising along his flank