Answer:
D. People who live in wealthier countries tend to consume more energy per person.
Explanation:
People in wealthy countries have access to many technologies such as phones, televisions, and house appliances like coffee makers. All of these use energy. Wealthy people also tend to have larger houses that require more electricity to light and heat/cool. People in developing countries do not have access to these technologies or large houses that consume large amounts of energy. Additionally, people in poverty are unable to afford the levels of energy that wealthy citizens can. For these reasons, wealthier countries consume more energy per person.
Simple version:
First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed).
Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
Type 1 stools are detached, hard lumps that be similar to nuts that are tough to pass. Type 3 stools are like a sausage, but with pops on the surface. Type 5 stools are mushy blobs with clear-cut ends that are passed effortlessly. Type 6 stools are cottony pieces with raggedy edges.
