Answer:
Extension
Explanation:
Polymerase chain reaction is given by Kary Mullis and it contains three steps denaturation, annealing, and extension. In denaturation, the temperature is raised to 96° c so that the DNA can denature.
In annealing, the temperature gets reduced to 55°c so that the primer can bind to the complementary sequence. In extension, Taq polymerase is used to extend the new strand.
This enzyme is isolated from thermophilic bacteria and the temperature here is raised to 72°c so that the enzyme can extend the primer and make the complementary strand. So the right answer is extension.
Answer:
Explanation:
When the cell increases in size, the volume increases faster than the surface area, because volume is cubed where surface area is squared. When there is more volume and less surface area, diffusion takes longer and is less effective.
Answer: NaDP+/NaDPH and D. glucose 6‑phosphate dehydrogenase
Explanation:
Pentose Phosphate pathway could be generally divided into oxidative and non-oxidative. The rate-limiting irreversible step in the oxidative step defined by the glucose-6-phosphate dehydrogenase which requires the reduction of NADP to NADPH. The enzyme is highly selective for the NADH and its activity is tightly dependent on the presence of the NADP