The answer to this question seems pretty specific and is probably in some assigned reading.. An example, however, might be if your hypothesis questioned a higher level of experimentation. For example, your experiment is to see the witness the result of a mixture of baking soda and vinegar. If your hypothesis was that the reaction would occur faster when heat was added, then you would have to further experiment to see wether or not that was true.
Transcription is important to genetic information. If there is an error during the transcription process, the DNA copies would not be accurate, causing the proteins to be improperly assembled, or not assembled at all.