<span>The primary desired therapeutic effect of mannitol is the reduction of intracranial pressure. This pressure, most likely caused by swelling on the brain, is generally caused by head traumas and can be life threatening. The administration of mannitol reduces this swelling thus decreasing the cranial pressure.</span>
They are ineffective against viral infections because viruses can not be treated with antibiotics.
I don’t know if this is a question but yes a controlled experiment allows you to isolate and test the hypothesis