If he studied a small sample it wouldn't be a representative sample. since he got such a large sample there is a chance most likely all organisms follow the rules of evolution.
Ageing is the word that describes all that change as the organism evolve and it also includes growth !
It effects the environment because it can cause unstability in the food web and biotic and abiotic factors
I believe Tidal volume is the correct answer
Every organism has a unique ecosystem within which it lives. This ecosystem is its natural habitat. This is where the basic needs of the organism to survive are met: food, water, shelter from the weather and place to breed its young. All organisms need to adapt to their habitat to be able to survive.