Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Answer: Option B) inability to digest proteins
Explanation:
The pancreas secretes trypsin, a proteolytic enzyme, that acts on proteins cleaving its peptide bonds, and eventually yielding peptones or polypeptides.
Thus, in the absence of pancreas protein digestion would be a problem
Answer:
A) A tentative statement used to guide a scientific investigation is called scientific hypothesis.
As hypothesis is a proposed details of a phenomena, it can guide us in one way or another to carry out the scientific investigation.
B) Makes prediction about future events is scientific hypothesis.
Scientific hypothesis bases on the observation made predicts about future events, which could be either true or false.
C) Can be tested many independent researches is a scientific theory.
A scientific theory is developed when it has been passed through many researches and had gained acceptance too.
D) Both Scientific theory and hypothesis are based on the observations of the natural phenomena.
Firstly, an observation is made based on natural phenomena, which leads to questions, then research or study is carried out to answer these questions leading to the formation of a hypothesis which upon successful testing forms a theory.
E) Scientific theory is a well-established highly reliable explanation.
It a highly sustained explanation based on facts of nature, that are confirmed via a lot of experiments.
Decomposer's role is to break down dead food. They basically eat it, then the dead food or the dead animal gets ground into the ground, and makes the soil good. Like worms, or fungi, mushrooms, etc. Rotten fruit is not really wasted because 1) the decomposers eat it, and 2) it makes the soil good
